2VON
Crystal structure of N-terminal domains of Human La protein complexed with RNA oligomer AUAAUUU
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-13 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 140.029, 44.471, 91.285 |
| Unit cell angles | 90.00, 114.35, 90.00 |
Refinement procedure
| Resolution | 43.000 - 2.100 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zh5 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALE |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.084 | 0.223 |
| Number of reflections | 27637 | |
| <I/σ(I)> | 7.5 | 4.1 |
| Completeness [%] | 90.4 | 92.2 |
| Redundancy | 1.7 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 0.2 M NaCl, 0.1 M phosphate citrate (pH 5.0), 22% (v/w) PEG 8000, 0.01 M taurine. |
