2VO4

Glutathione transferase from Glycine max

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 41 21 2
Unit cell lengths	91.375, 91.375, 111.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.710 - 1.750
R-factor	0.197
Rwork	0.194
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1oyj
RMSD bond length	0.015
RMSD bond angle	1.634
Data reduction software	HKL
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.710	1.780
High resolution limit [Å]	1.750	1.750
Rmerge	0.050	0.520
Number of reflections	48045
<I/σ(I)>		4.1
Completeness [%]	98.9	100
Redundancy	42

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				PEG