2VNX
Crystal structure of soybean ascorbate peroxidase mutant W41A after exposure to a high dose of x-rays
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-03 |
| Detector | ADSC CCD |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 82.002, 82.002, 75.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.800 - 1.500 |
| R-factor | 0.177 |
| Rwork | 0.175 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ggn |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.152 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.800 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.090 | 0.660 |
| Number of reflections | 34661 | |
| <I/σ(I)> | 19.5 | 3.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.18 | 9.89 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.3 | pH 8.3 |
