2VNS
Crystal Structure of the Membrane Proximal Oxidoreductase Domain of Human Steap3, the Dominant Ferric Reductase of the Erythroid Transferrin Cycle
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.07149, 0.82654, 1.07199, 1.00545, 1.1271 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.686, 66.812, 143.366 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.205 |
| Rwork | 0.203 |
| R-free | 0.24400 |
| Structure solution method | MIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.050 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.050 | 0.240 |
| Number of reflections | 25300 | |
| <I/σ(I)> | 16.1 | 3.3 |
| Completeness [%] | 96.8 | 98.3 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 0.01M FECL3, 40-100MM NA3.CITRATE AND 4% V/V JEFFAMINE M600 PH5.6 |
