2VNJ

X-RAY STRUCTURE OF THE FERREDOXIN-NADP(H) REDUCTASE FROM RHODOBACTER CAPSULATUS IN COMPLEX WITH NADP. FORM I AT 2.13 ANGSTROMS RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR571
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	2005-03-15
Detector	MARRESEARCH
Spacegroup name	P 31 2 1
Unit cell lengths	68.720, 68.720, 128.150
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.280 - 2.130
R-factor	0.237
Rwork	0.237
R-free	0.27100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bgi
RMSD bond length	0.014
RMSD bond angle	1.900
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.290	2.260
High resolution limit [Å]	2.130	2.130
Rmerge	0.080	0.540
Number of reflections	43856
<I/σ(I)>	11.1	2.1
Completeness [%]	99.4	98.1
Redundancy	3.2	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1