2VNI

X-RAY STRUCTURE OF THE FERREDOXIN-NADP(H) REDUCTASE FROM RHODOBACTER CAPSULATUS IN COMPLEX WITH 2P-AMP AT 2.37 ANGSTROMS RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	100
Collection date	2005-06-10
Spacegroup name	P 31 2 1
Unit cell lengths	121.116, 121.116, 50.574
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.560 - 2.240
R-factor	0.229
Rwork	0.229
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bgi
RMSD bond length	0.010
RMSD bond angle	1.500
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.560	2.730
High resolution limit [Å]	2.240	2.240
Rmerge	0.100	0.320
Number of reflections	24368
<I/σ(I)>	11.8	4.8
Completeness [%]	98.9	97.4
Redundancy	5.9	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1