2VNG
Family 51 carbohydrate binding module from a family 98 glycoside hydrolase produced by Clostridium perfringens in complex with blood group A-trisaccharide ligand.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002 |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 69.683, 98.754, 49.241 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.800 - 1.400 |
| R-factor | 0.187 |
| Rwork | 0.184 |
| R-free | 0.23500 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.881 |
| Data reduction software | CrystalClear |
| Data scaling software | d*TREK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.800 | 1.430 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.030 | |
| Number of reflections | 57807 | |
| <I/σ(I)> | 18.1 | |
| Completeness [%] | 89.3 | 68.8 |
| Redundancy | 3.41 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | pH 7.5 |
