2VND
The N69Q mutant of Vibrio cholerae endonuclease I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector | RIGAKU R-AXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.265, 64.411, 75.639 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.700 |
| R-factor | 0.2 |
| Rwork | 0.197 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g7e |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.520 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0021) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.060 | 0.360 |
| Number of reflections | 22098 | |
| <I/σ(I)> | 12.8 | 2.2 |
| Completeness [%] | 99.3 | 99.1 |
| Redundancy | 2.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.1 M SODIUM ACETATE, 0.3 M AMMONIUM ACETATE, 10 MM MAGNESIUM SULPHATE, 26% PEG8000, pH 8.0 |
