2VNC
Crystal structure of Glycogen Debranching enzyme TreX from Sulfolobus solfataricus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2007-11-14 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 136.077, 136.077, 173.545 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.910 - 3.000 |
| R-factor | 0.218 |
| Rwork | 0.215 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bf2 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.372 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 3.000 | 2.700 |
| Rmerge | 0.070 | 0.410 |
| Number of reflections | 51429 | |
| <I/σ(I)> | 10.3 | 2.75 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 14.5 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 2.2M AMMONIUM PHOSPHATE, 0.1M TRIS-HCL (PH 8.5) |
