2VNA
Structure of Human Zinc-binding Alcohol Dehydrogenase 1 (ZADH1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-09 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.335, 83.505, 72.585 |
| Unit cell angles | 90.00, 108.43, 90.00 |
Refinement procedure
| Resolution | 34.430 - 2.170 |
| R-factor | 0.192 |
| Rwork | 0.189 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vj1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.177 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.091 | 0.780 |
| Number of reflections | 148662 | |
| <I/σ(I)> | 16.7 | 1.9 |
| Completeness [%] | 98.4 | 89.5 |
| Redundancy | 7.1 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 2.2M SODIUM MALONATE PH 7.0 |
