2VN7
Glycoside Hydrolase Family 15 Glucoamylase from Hypocrea jecorina
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-5 |
| Synchrotron site | MAX II |
| Beamline | I911-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-20 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 216.756, 48.490, 50.562 |
| Unit cell angles | 90.00, 90.49, 90.00 |
Refinement procedure
| Resolution | 16.960 - 1.900 |
| R-factor | 0.20365 |
| Rwork | 0.201 |
| R-free | 0.25331 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vn4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.218 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 16.980 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.080 | 0.400 |
| Number of reflections | 41660 | |
| <I/σ(I)> | 19.7 | 3 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 5.4 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 25% PEG3350, 0.10M CACL2, 0.10M BIS-TRIS PH 5.5 |
