2VN3
Nitrite Reductase from Alcaligenes xylosoxidans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | H 3 |
| Unit cell lengths | 89.451, 89.451, 144.473 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.140 - 2.350 |
| R-factor | 0.167 |
| Rwork | 0.165 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oe1 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.539 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.100 | 2.410 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.050 | 0.320 |
| Number of reflections | 17939 | |
| <I/σ(I)> | 25.9 | 4.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.4 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 100 MM MES PH 6.5, 10 MM ZNSO4 AND 25 % PEG-MME 550 |
