2VML
The monoclinic structure of phycocyanin from Gloeobacter violaceus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-08 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.877, 142.040, 115.313 |
| Unit cell angles | 90.00, 107.65, 90.00 |
Refinement procedure
| Resolution | 109.760 - 2.400 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vjr |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.056 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.440 | 2.420 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.110 | 0.330 |
| Number of reflections | 102846 | |
| <I/σ(I)> | 8.94 | 1.71 |
| Completeness [%] | 98.9 | 93.7 |
| Redundancy | 3.63 | 3.08 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8% PEG 4000, 0.1 M NA ACETATE PH 4.6 MIXED WITH IN EQUAL VOLUME OF 10MG/ML PROTEIN SOLUTION, HANGING DROP VAPOUR DIFFUSION |
