2VMJ
Type 1 Copper-Binding Loop of Nitrite Reductase mutant: 130- CAPEGMVPWHVVSGM-144 to 130-CTPHPFM-136
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 |
| Unit cell lengths | 89.813, 89.813, 143.099 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.530 - 2.500 |
| R-factor | 0.184 |
| Rwork | 0.181 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oe1 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.536 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.500 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.060 | 0.190 |
| Number of reflections | 14625 | |
| <I/σ(I)> | 9.4 | 2.4 |
| Completeness [%] | 98.4 | 99.9 |
| Redundancy | 1.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 50 MM HEPES PH 7.0, 5 MM ZNCL2 AND 10 % PEG 6000 |
