2VMC
Structure of the complex of discoidin II from Dictyostelium discoideum with N-acetyl-galactosamine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-11-30 |
Detector | ADSC CCD |
Spacegroup name | H 3 2 |
Unit cell lengths | 82.520, 82.520, 261.600 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.600 - 1.900 |
R-factor | 0.159 |
Rwork | 0.157 |
R-free | 0.19800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vm9 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.412 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.200 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.100 | 0.340 |
Number of reflections | 27554 | |
<I/σ(I)> | 6.5 | 2.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 25% PEG5550MME, 100 MM HEPES PH 7.5 |