2VM1

Crystal structure of barley thioredoxin h isoform 1 crystallized using ammonium sulfate as precipitant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Spacegroup name	C 1 2 1
Unit cell lengths	120.881, 33.533, 131.048
Unit cell angles	90.00, 112.31, 90.00

Refinement procedure

Resolution	121.270 - 1.700
R-factor	0.182
Rwork	0.181
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.181
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.500	1.790
High resolution limit [Å]	1.700	1.700
Rmerge	0.070	0.480
Number of reflections	53797
<I/σ(I)>	9	1.6
Completeness [%]	99.0	96
Redundancy	4.8	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1