2VM1
Crystal structure of barley thioredoxin h isoform 1 crystallized using ammonium sulfate as precipitant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.881, 33.533, 131.048 |
| Unit cell angles | 90.00, 112.31, 90.00 |
Refinement procedure
| Resolution | 121.270 - 1.700 |
| R-factor | 0.182 |
| Rwork | 0.181 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.181 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.500 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.070 | 0.480 |
| Number of reflections | 53797 | |
| <I/σ(I)> | 9 | 1.6 |
| Completeness [%] | 99.0 | 96 |
| Redundancy | 4.8 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
