2VLO
K97A mutant of E9 DNase domain in complex with Im9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 110 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.382, 78.618, 40.217 |
| Unit cell angles | 90.00, 99.36, 90.00 |
Refinement procedure
| Resolution | 27.950 - 1.800 |
| R-factor | 0.177 |
| Rwork | 0.174 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1emv |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.811 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0001) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.060 | 0.250 |
| Number of reflections | 19052 | |
| <I/σ(I)> | 13.9 | 3.9 |
| Completeness [%] | 91.9 | 91.9 |
| Redundancy | 2.8 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
