2VLG

KinA PAS-A domain, homodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-04-12
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	53.568, 55.516, 78.879
Unit cell angles	90.00, 109.01, 90.00

Refinement procedure

Resolution	44.000 - 1.700
R-factor	0.2
Rwork	0.198
R-free	0.24300
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.018
RMSD bond angle	1.761
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELX
Refinement software	REFMAC (5.3.0037)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.750	1.740
High resolution limit [Å]	1.710	1.710
Rmerge	0.040	0.420
Number of reflections	42220
<I/σ(I)>	24.9	1.9
Completeness [%]	88.0	56
Redundancy	2.9	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	20 DEGREES CELSIUS, HANGING-DROP VAPOR DIFFUSION: 1 MICROLITER KINA (7 MG/ML IN 25 MM TRIS, PH 8, 100 MM NACL PLUS 1 MICROLITER WELL SOLUTION (13 TO 15% (W/V) PEG 10,000, 0.1 M AMMONIUM ACETATE, 0.1 M BIS-TRIS, PH 5.5); CRYSTALS APPEARED AFTER ABOUT ONE DAY AND GREW TO A FINAL SIZE OF ABOUT 80 X 80 X 100 MICROMETERS WITHIN FOUR DAYS; CRYO-PROTECTION: STEPWISE TRANSFER INTO WELL SOLUTION SUPPLEMENTED WITH UP TO 25% (V/V) GLYCEROL.