2VLA
Crystal structure of restriction endonuclease BpuJI recognition domain in complex with cognate DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 56.292, 167.513, 43.846 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 83.620 - 1.300 |
| R-factor | 0.14 |
| Rwork | 0.138 |
| R-free | 0.16600 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.099 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.990 | 1.370 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.080 | 0.310 |
| Number of reflections | 101561 | |
| <I/σ(I)> | 4.7 | 2.3 |
| Completeness [%] | 98.0 | 98.2 |
| Redundancy | 10.8 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.6 | 0.2 M AMMONIUM TARTRATE, 20% PEG3350, pH 6.6 |
