2VL9
Oxidized form of human peroxiredoxin 5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-11 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 75.870, 75.870, 193.254 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.000 - 2.700 |
| R-factor | 0.1863 |
| Rwork | 0.184 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oc3 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.636 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.050 | 0.460 |
| Number of reflections | 18076 | |
| <I/σ(I)> | 17.9 | 3.4 |
| Completeness [%] | 98.1 | 99.6 |
| Redundancy | 4.05 | 4.05 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | PEG 8000 20% SODIUM CACODYLATE 0.1 M PH 6.5 SODIUM ACETATE 0.1 M 6-AMINOCAPROIC ACID 3% W/V |
