2VL5
Structure of anti-collagen type II FAb CIIC1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.600, 78.000, 99.500 |
| Unit cell angles | 90.00, 83.10, 90.00 |
Refinement procedure
| Resolution | 24.420 - 2.100 |
| R-factor | 0.216 |
| Rwork | 0.212 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fsk |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.696 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.120 | 0.400 |
| Number of reflections | 191077 | |
| <I/σ(I)> | 10.66 | 4.04 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 0.2 SODIUM-FORMATE, 20 % PEG 3350, PH 7.2 HANGING DROP |
