2VL1
Crystal structure of beta-alanine synthase from Saccharomyces kluyveri in complex with a gly-gly peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.835, 218.370, 81.499 |
| Unit cell angles | 90.00, 91.85, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.150 |
| R-factor | 0.205 |
| Rwork | 0.202 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v8g |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.197 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.130 | 0.450 |
| Number of reflections | 89524 | |
| <I/σ(I)> | 8.7 | 2.2 |
| Completeness [%] | 95.2 | 97.1 |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.75 | PEG 6000, TRIS PH 8.5, BICINE PH 9.0, LICL |
