2VKY

Headbinding Domain of Phage P22 Tailspike C-Terminally Fused to Isoleucine Zipper pIIGCN4 (Chimera I)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU H2B
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2005-09-08
Detector	MARRESEARCH
Spacegroup name	P 63
Unit cell lengths	57.810, 57.810, 107.120
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.050
R-factor	0.147
Rwork	0.145
R-free	0.18700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1LKT 1ebo
RMSD bond length	0.014
RMSD bond angle	1.450
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.700	2.200
High resolution limit [Å]	2.050	2.050
Number of reflections	12716
<I/σ(I)>	31.66	13.6
Completeness [%]	99.6	99.5
Redundancy	4.2	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5		VAPOR DIFFUSION, HANGING DROP. PROTEIN: CONC. 8.2 MG/ML,BUFFER 50MM HEPES, PH6.5; RESERVOIR: 20% ISOPROPANOL, 0.1M NA-ACETATE, PH4.6, 0.2M CACL2; DROPLET 2 MICROL: 2 MICROL.CRYO:30% GLYCEROL.