2VKT
HUMAN CTP SYNTHETASE 2 - GLUTAMINASE DOMAIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-02 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.200, 73.100, 50.500 |
| Unit cell angles | 90.00, 95.60, 90.00 |
Refinement procedure
| Resolution | 19.980 - 2.500 |
| R-factor | 0.209 |
| Rwork | 0.207 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vco |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.271 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0040) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.100 | 0.550 |
| Number of reflections | 13231 | |
| <I/σ(I)> | 13.38 | 3.5 |
| Completeness [%] | 99.5 | 99.6 |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.1 M HEPES PH 7, 26% POLYACRYLIC ACID 5100, 0.02 M MGCL2 |
