2VK5
THE STRUCTURE OF CLOSTRIDIUM PERFRINGENS NANI SIALIDASE AND ITS CATALYTIC INTERMEDIATES
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 96.892, 69.019, 72.808 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 0.970 |
| Rwork | 0.115 |
| R-free | 0.12600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sll |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.048 |
| Data reduction software | MOSFLM |
| Phasing software | CNS |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.020 |
| High resolution limit [Å] | 0.970 | 0.970 |
| Rmerge | 0.070 | 0.330 |
| Number of reflections | 281317 | |
| <I/σ(I)> | 15.6 | 3.6 |
| Completeness [%] | 98.0 | 95 |
| Redundancy | 4.6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
