2VJW
crystal structure of the second GAF domain of DevS from Mycobacterium smegmatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 294 |
| Detector technology | CCD |
| Collection date | 2007-01-14 |
| Detector | ADSC CCD |
| Spacegroup name | P 61 |
| Unit cell lengths | 73.232, 73.232, 48.542 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.240 - 2.000 |
| R-factor | 0.21 |
| Rwork | 0.208 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.273 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.380 |
| Number of reflections | 10102 | |
| <I/σ(I)> | 31.7 | 5.3 |
| Completeness [%] | 99.5 | 97.1 |
| Redundancy | 7.2 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
