2VJP

Formyl-CoA transferase mutant variant W48F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-09-29
Detector	MARRESEARCH
Spacegroup name	I 4
Unit cell lengths	152.716, 152.716, 99.448
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.950
R-factor	0.168
Rwork	0.166
R-free	0.20100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1p5h
RMSD bond length	0.009
RMSD bond angle	1.107
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.250	2.060
High resolution limit [Å]	1.950	1.950
Rmerge	0.130	0.530
Number of reflections	80837
<I/σ(I)>	8.8	2.7
Completeness [%]	97.9	99.5
Redundancy	4.2	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1