2VJP
Formyl-CoA transferase mutant variant W48F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-29 |
| Detector | MARRESEARCH |
| Spacegroup name | I 4 |
| Unit cell lengths | 152.716, 152.716, 99.448 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.950 |
| R-factor | 0.168 |
| Rwork | 0.166 |
| R-free | 0.20100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p5h |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.107 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.250 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.130 | 0.530 |
| Number of reflections | 80837 | |
| <I/σ(I)> | 8.8 | 2.7 |
| Completeness [%] | 97.9 | 99.5 |
| Redundancy | 4.2 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
