2VIX
Methylated Shigella flexneri MxiC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 294 |
| Detector technology | CCD |
| Collection date | 2007-07-04 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.791, 102.572, 122.638 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.400 - 2.850 |
| R-factor | 0.244 |
| Rwork | 0.244 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.821 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | TNT (5.13.1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.400 | 3.000 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.110 | 0.490 |
| Number of reflections | 84697 | |
| <I/σ(I)> | 10.7 | 2.4 |
| Completeness [%] | 98.6 | 93.3 |
| Redundancy | 3.2 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2M NAACETATE, 0.1 BISTRIS PROPANE PH 7.5, 20% PEG3350 |
