2VII
PspF1-275-Mg-AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-23 |
| Detector | ADSC CCD |
| Spacegroup name | P 65 |
| Unit cell lengths | 113.607, 113.607, 39.358 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 98.390 - 2.850 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bjw |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 98.390 | 3.000 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.140 | 0.360 |
| Number of reflections | 6998 | |
| <I/σ(I)> | 4.7 | 1.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.7 | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.1M TRIS-HCL PH 8.0, 2.0M AMMONIUM FORMATE, 10% MPD |
