2VIC
CRYSTAL STRUCTURE OF THE ISHP608 TRANSPOSASE IN COMPLEX with Left end 26- mer DNA and manganese
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 74.206, 50.261, 115.625 |
| Unit cell angles | 90.00, 105.60, 90.00 |
Refinement procedure
| Resolution | 19.470 - 2.350 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vih |
| RMSD bond length | 0.007 |
| RMSD bond angle | 2.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.370 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.080 | 0.440 |
| Number of reflections | 17657 | |
| <I/σ(I)> | 12.3 | 1.5 |
| Completeness [%] | 94.7 | 86.1 |
| Redundancy | 2.7 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2 M SODIUM FORMATE, 15-20% PEG 3350, pH 7.5 |
