2VHZ

Crystal structure of holo L-alanine dehydrogenase from Mycobacterium tuberculosis in the closed conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-12
Detector	ADSC CCD
Spacegroup name	P 21 3
Unit cell lengths	140.991, 140.991, 140.991
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.040
R-factor	0.166
Rwork	0.164
R-free	0.20400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.464
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.700	2.150
High resolution limit [Å]	2.040	2.040
Rmerge	0.100	0.580
Number of reflections	359770
<I/σ(I)>	10.1	2.8
Completeness [%]	100.0	100
Redundancy	6	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8		pH 8