2VHI
Crystal structure of a pyrimidine degrading enzyme from Drosophila melanogaster
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 278.863, 95.048, 199.312 |
| Unit cell angles | 90.00, 125.82, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.300 |
| R-factor | 0.229 |
| Rwork | 0.226 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.255 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.170 | 0.380 |
| Number of reflections | 54793 | |
| <I/σ(I)> | 6.4 | 2.1 |
| Completeness [%] | 86.0 | 81.2 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.2 | PEG 3350, PHOSPHATE/CITRATE PH 4.2, NACL |
