2VHE
PglD-CoA complex: An acetyl transferase from Campylobacter jejuni
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-08 |
| Detector | ADSC CCD |
| Spacegroup name | H 3 |
| Unit cell lengths | 99.250, 99.250, 143.027 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 1.800 |
| R-factor | 0.191 |
| Rwork | 0.190 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2npo |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.604 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.070 | 0.460 |
| Number of reflections | 48692 | |
| <I/σ(I)> | 11.4 | 2.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M SODIUM CITRATE PH 5.6, 0.16 M SODIUM TARTRATE AND 2.0 M AMMONIUM SULFATE |
