2VH7
Crystal structure of human common-type acylphosphatase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU-MSC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.577, 47.235, 57.261 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.440 - 1.450 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2acy |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.325 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.700 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.030 | 0.290 |
| Number of reflections | 20888 | |
| <I/σ(I)> | 19.8 | 2.9 |
| Completeness [%] | 99.1 | 95.4 |
| Redundancy | 4.28 | 2.97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 10%(V/V) 2-PROPANOL, 20%(W/V) PEG 4000, 0.1 M NA HEPES BUFFER PH 7.5 |
