2VH5

CRYSTAL STRUCTURE OF HRAS(G12V) - ANTI-RAS FV (disulfide free mutant) COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-12-08
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	75.863, 85.403, 63.084
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.180 - 2.700
R-factor	0.215
Rwork	0.211
R-free	0.29100
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.011
RMSD bond angle	1.387
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	42.180
High resolution limit [Å]	2.700
Rmerge	0.130
Number of reflections	11682
<I/σ(I)>	12.4
Completeness [%]	99.5
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.8		PROTEIN WAS CRYSTALLIZED FROM 17-18 % PEG3350, 320 MM ZINC ACETATE, 100 MM SODIUM CACODYLATE, PH 5.8, 0.03 % DICHLOROMETHANE