2VH4
Structure of a loop C-sheet serpin polymer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-09-24 |
| Detector | RIGAKU-MSC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.895, 68.669, 137.651 |
| Unit cell angles | 90.00, 93.45, 90.00 |
Refinement procedure
| Resolution | 137.360 - 2.450 |
| R-factor | 0.196 |
| Rwork | 0.192 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pef |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.989 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 137.300 | |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.010 | 0.050 |
| Number of reflections | 28981 | |
| <I/σ(I)> | 14.4 | 2.2 |
| Completeness [%] | 98.3 | 91.8 |
| Redundancy | 3.4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.8 | 100MM BIS-TRIS, PH5.8, 14% (W/V) PEG 5000, MONOMETHYL ETHER |
