2VH1
Crystal structure of bacterial cell division protein FtsQ from E.coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 65 |
| Unit cell lengths | 147.844, 147.844, 69.259 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.350 - 2.700 |
| R-factor | 0.24 |
| Rwork | 0.237 |
| R-free | 0.28800 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.639 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.100 | 0.350 |
| Number of reflections | 21005 | |
| <I/σ(I)> | 9.9 | 2.3 |
| Completeness [%] | 98.3 | 98.3 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 33 MM N-OCTYL-B-D-GLUCOSIDE, 50 MM TRIS PH8.5, 8% PEG550MME, 8% PEG20K, 1.2 M NAFORMATE |
