2VGY
Crystal structure of the Yersinia enterocolitica Type III Secretion Translocator Chaperone SycD (alternative dimer)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-30 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 90.638, 90.638, 54.205 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.247 - 2.600 |
| Rwork | 0.213 |
| R-free | 0.23430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vgx |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.200 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.120 | 0.600 |
| Number of reflections | 8163 | |
| <I/σ(I)> | 20 | 3.7 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 7.2 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 277 | 40% PEG400, 5% PEG3350, 0.1M SODIUM ACTATE PH 5.5 AT 4 DEGREES CELSIUS |
