2VGX

Structure of the Yersinia enterocolitica Type III Secretion Translocator Chaperone SycD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-02-15
Detector	ADSC CCD
Spacegroup name	C 1 2 1
Unit cell lengths	107.247, 32.979, 96.731
Unit cell angles	90.00, 122.34, 90.00

Refinement procedure

Resolution	45.310 - 1.950
R-factor	0.178
Rwork	0.175
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fo7
RMSD bond length	0.015
RMSD bond angle	1.443
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.300	2.050
High resolution limit [Å]	1.950	1.950
Rmerge	0.090	0.310
Number of reflections	21282
<I/σ(I)>	13.1	3.8
Completeness [%]	99.6	99.8
Redundancy	4	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1			277	0.1M TRI-SODIUM CITRATE, 20% PEG 4000, 20% 2-PROPANOL AT 4 DEGREES CELSIUS
1			277	0.1M TRI-SODIUM CITRATE, 20% PEG 4000, 20% 2-PROPANOL AT 4 DEGREES CELSIUS