2VGU

Crystal structure of E53QbsSHMT with L-serine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	100
Wavelength(s)	1.54
Spacegroup name	P 21 21 2
Unit cell lengths	61.304, 106.472, 57.289
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.030 - 1.800
R-factor	0.168
Rwork	0.166
R-free	0.20000
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	NONE
RMSD bond length	0.011
RMSD bond angle	1.233
Refinement software	REFMAC (5.2.0019)

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		50% MPD, 0.1 M HEPES, PH 7.5, 0.2 MM EDTA, 5 MM 2-MECAPTOETHANOL, 10 MM L-SER