2VGT
Crystal structure of E53QbsSHMT with glycine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 61.282, 106.301, 57.317 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.650 - 1.860 |
| R-factor | 0.163 |
| Rwork | 0.159 |
| R-free | 0.20200 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1kl1 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.208 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.060 | 0.330 |
| Number of reflections | 304348 | |
| <I/σ(I)> | 18.2 | 4 |
| Completeness [%] | 99.2 | 94.4 |
| Redundancy | 3.8 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 50 % MPD, 0.1 M HEPES, 0.2 MM EDTA, 5 MM 2-MECAPTOETHANOL, 10 MM GLYCINE, pH 7.5 |
