2VGR
Structure of the WT-Phycoerythrobilin Synthase PebS from the Cyanophage P-SSM2 in Complex with the bound Substrate Biliverdin IXa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-19 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.990, 70.040, 81.670 |
| Unit cell angles | 75.53, 70.74, 71.13 |
Refinement procedure
| Resolution | 47.620 - 2.100 |
| R-factor | 0.23 |
| Rwork | 0.226 |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vck |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.516 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 2.300 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.080 | 0.300 |
| Number of reflections | 60138 | |
| <I/σ(I)> | 12.8 | 5.3 |
| Completeness [%] | 97.5 | 96.9 |
| Redundancy | 4.3 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
