2VFY
AKAP18 delta central domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-30 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 |
| Unit cell lengths | 42.417, 42.417, 96.906 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 97.130 - 1.800 |
| R-factor | 0.207 |
| Rwork | 0.206 |
| R-free | 0.23800 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.252 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.130 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.040 | 0.270 |
| Number of reflections | 18073 | |
| <I/σ(I)> | 17.7 | 3.1 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 2.73 | 2.71 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M TRIS HCL PH 8, 10 % PEG 8K, 7 MM DTT |
