2VFU
Alditol Oxidase from Streptomyces coelicolor A3(2): Complex with Mannitol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Collection date | 2007-03-30 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.964, 65.931, 58.850 |
| Unit cell angles | 90.00, 94.49, 90.00 |
Refinement procedure
| Resolution | 19.870 - 1.900 |
| R-factor | 0.155 |
| Rwork | 0.153 |
| R-free | 0.19900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vfr |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.628 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.100 | 0.540 |
| Number of reflections | 32193 | |
| <I/σ(I)> | 12.4 | 1.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | pH 6.5 |
