2VFT
Alditol Oxidase from Streptomyces coelicolor A3(2): Complex with Sorbitol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-12 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 107.185, 66.622, 59.134 |
Unit cell angles | 90.00, 95.09, 90.00 |
Refinement procedure
Resolution | 33.310 - 1.600 |
R-factor | 0.154 |
Rwork | 0.152 |
R-free | 0.17900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vfr |
RMSD bond length | 0.019 |
RMSD bond angle | 1.650 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 |
Phasing software | CCP4 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.900 | 1.700 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.050 | 0.240 |
Number of reflections | 54653 | |
<I/σ(I)> | 21.5 | 5.2 |
Completeness [%] | 99.9 | 100 |
Redundancy | 4.8 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | pH 6.5 |