2VFS
Alditol Oxidase from Streptomyces coelicolor A3(2): Complex with Xylitol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-12 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.961, 67.078, 59.814 |
| Unit cell angles | 90.00, 94.83, 90.00 |
Refinement procedure
| Resolution | 33.540 - 1.600 |
| R-factor | 0.175 |
| Rwork | 0.173 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vfr |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.561 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.700 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.050 | 0.500 |
| Number of reflections | 53338 | |
| <I/σ(I)> | 9 | 3 |
| Completeness [%] | 94.1 | 85.1 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | pH 6.5 |
