2VFL
AKAP18 delta central domain - CMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-30 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 |
| Unit cell lengths | 42.968, 42.968, 97.974 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 98.060 - 2.250 |
| R-factor | 0.226 |
| Rwork | 0.225 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | APO STRUCTURE |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.721 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 98.060 | 2.310 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.090 | 0.300 |
| Number of reflections | 9559 | |
| <I/σ(I)> | 13.6 | 5.2 |
| Completeness [%] | 99.5 | 98.4 |
| Redundancy | 4.1 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M TRIS HCL PH 8, 10 % PEG 8K, 7 MM DTT |
