2VFK
AKAP18 delta central domain - AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-30 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 |
| Unit cell lengths | 42.233, 42.233, 97.723 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 97.590 - 1.500 |
| R-factor | 0.163 |
| Rwork | 0.161 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | APO STRUCTURE |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.590 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.590 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.040 | 0.320 |
| Number of reflections | 31159 | |
| <I/σ(I)> | 18.2 | 2.5 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 2.7 | 2.71 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M TRIS HCL PH 8, 10 % PEG 8K, 7 MM DTT |
