2VFJ

Structure of the A20 Ovarian Tumour (OTU) domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-10
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	84.964, 83.023, 164.940
Unit cell angles	90.00, 98.08, 90.00

Refinement procedure

Resolution	50.286 - 3.200
R-factor	0.2064
Rwork	0.204
R-free	0.24290
Structure solution method	MAD
Starting model (for MR)	NONE
RMSD bond length	0.011
RMSD bond angle	1.377
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELXCDE
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.370
High resolution limit [Å]	3.200	3.200
Rmerge	0.080	0.560
Number of reflections	36226
<I/σ(I)>	15.9	2.6
Completeness [%]	96.0	96
Redundancy	4.2	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				1.3-1.6 M MAGNESIUM SULPHATE, 0.1 M MES [PH 6.5-6.9]