2VFB
The structure of Mycobacterium marinum arylamine N-acetyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 51.526, 51.526, 175.299 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.060 - 2.000 |
| R-factor | 0.191 |
| Rwork | 0.189 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gx3 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.843 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.500 | 2.110 |
| High resolution limit [Å] | 1.450 | 2.000 |
| Rmerge | 0.090 | 0.480 |
| Number of reflections | 16884 | |
| <I/σ(I)> | 6.7 | 1.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.9 | 9.24 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 200MM CACL2, 100MM HEPES, PH7.5, 28% POLY(ETHYLENE GLYCOL) 400 |
